No abstracts in this session.

No abstracts in this session.

• ES1 Gene-Electrotransfer Applications in Veterinary Oncology
  Luigi Aurisicchio, Antonella Conforti, Joseph A. Impellizeri

No abstracts in this session.

No abstracts in this session.

• 09:00–09:30 PT1 Unresolved Issues in Reversible and Irreversible Electroporation
  Damijan Miklavčič
• 09:30–10:00 PT2 Reversible electroporation by pulsed electric field - Opportunities and challenges for food and agriculture
  Federico Gómez Galindo

• 10:30–10:45 O1 From Gene Editing to Cytokine Dynamics: Microfluidic Technologies for Cellular Perturbation and Response Analysis
  Horacio Espinosa
• 10:45–11:00 O2 Engineering Therapeutic Extracellular Vesicles through Nanoscale Electroporation
  Natalia Higuita-Castro
• 11:00–11:15 O3 Geometric Confinement Reshapes Intracellular Cargo Transport in Nanopore Electroporation
  Emily McCorcle, Matthew Manion, Xiaoqian Wang, Cheyenne Meeks, Nyvia Lyles, Guanren Tao, Sasha Cai Lesher Perez, Albert Liu
• 11:15–11:30 O4 Engineering the Skin to Heal the Body: Tissue Nanotransfection as a Non-Viral Therapeutic Platform
  Daniel Gallegeo-Perez
• 11:30–11:45 O5 Engineering Nanostraws and Nanopores for Precision Transfection: A Non-Viral Route to β-Cell Epigenetic Editing
  Christelle Prinz
• 11:45–12:00 O6 In Situ Spatiotemporal Patterning By Integrating Nanopore Electroporation into Microfluidic Systems
  Emily McCorkle, Albert Liu, Sasha Cai Lesher Perez

• 10:30–10:45 O7 Strain-dependent response of yeasts to pulsed electric fields and its impact on nitrogen fraction recovery
  Javier Marín-Sánchez, Alejandro Berzosa, Ignacio Álvarez, Elisa Luengo, Javier Raso, Ana Cristina Sánchez-Gimeno
• 10:45–11:00 O8 Repeated extraction of cytoplasmatic components from filamentous fungi using pulsed electric fields (PEF)
  Benjamin Schmiedl, Felix Schottroff
• 11:00–11:15 O9 Pulsed electric field-mediated autolysis accelerates yeast extract production and reshapes amino acid profiles
  Alejandro Berzosa, Javier Marín-Sánchez, Ignacio Álvarez, Juan Manuel Martínez, Ana Cristina Sánchez-Gimeno, Javier Raso
• 11:15–11:30 O10 Deep Eutectic Solvent Extraction of Carotenoids from Rhodotorula glutinis Assisted by Pulsed Electric Fields
  Juan Manuel Martínez, Alejandro Berzosa, Javier Raso
• 11:30–11:45 O11 Intensification of fungal fermentations using pulsed electric fields in upstream and downstream processing
  Felix Schottroff, Benjamin Schmiedl
• 11:45–12:00 O12 Osmolarity as a tunable parameter to shape membrane resealing windows in microalgae pulsed electric field processing
  Byron Patricio Perez Simba, Beatrice Vuilleumier-Kaufmann, Iris Haberkorn, Alexander Mathys

• 10:30–10:45 O13 Isolated Phrenic and Vagus Nerve Stimulation and Stunning Induced by Electroporation Pulses
  Matej Reberšek, Weston Upchurch, Rok Šmerc, Daniel C. Sigg, Lea Rems, Damijan Miklavčič, Paul A. Iaizzo
• 10:45–11:00 O14 When More Is Not Deeper: Limited Incremental Lesion Depth from Radiofrequency Combined with Optimized Pulsed Field Ablation in an Acute Swine Model
  Lakshya Mittal, Chris Lafean, Hannah J. Loughlin, Ammar M. Killu, Suraj Kapa, Jeffrey M. Fish
• 11:00–11:15 O15 Unipolar Electrogram Morphological Changes as Predictors of Acute Lesion Volume in Pulsed Field Ablation
  Gerard Amorós-Figueras, Álvaro Borachok, Sergi Casabella-Ramon, Zoraida Moreno-Weidmann, Tomás Garcia-Sanchez, Antoni Ivorra, Jose M. Guerra
• 11:15–11:30 O16 Survival Dynamics Model for Lesion Size Estimation in Cardiac Pulsed-Field Ablation
  Sachin Gunda, Cristian Barrios Espinosa, Arthur Boileve, Jonas Butz, Teresa Schiatti, Emanuelle Costa, Simeon Pieper, Jörg Reinhardt, Peter Kohl, Fritz Koch, Remi Peyronnet, Axel Loewe
• 11:30–11:45 O17 A Multiphysics Framework for Differentiating Apoptotic and Viable Cardiac Tissue During Irreversible Electroporation
  Mayank Kumar, Rochish M Thaokar
• 11:45–12:00 O18 Impact of Contact Force and Electrode Configuration on Electric Field Distribution in Focal Pulsed Field Ablation: a Computational Study on a Realistic Porcine Geometry
  Luca Pasini, Gerard Amorós-Figueras, Zoraida Moreno-Weidmann, David Vilades Medel, Aurel Neic, Edward J. Vigmond, Antoni Ivorra, Jose M. Guerra, Luca Gerardo-Giorda, Argyrios Petras

No abstracts in this session.

No abstracts in this session.

• 13:30–13:45 O31 Contraction-Free Gene Electrotransfer Improves Expression in Skeletal Muscle
  Michael Francis, Alex Otten, Nadezhda Korostyleva, Jacob Hensley, Lina Sabkhangulova, Anna Bulysheva
• 13:45–14:00 O32 In vivo mRNA electrotransfer to murine tumors: optimization and antitumor efficacy
  Ajda Medved, Ursa Lampreht Tratar, Matjaz Peterka, Urban Bezeljak, Nastja Stemberger, Tjasa Marusic, Maja Cemazar
• 14:00–14:15 O33 Computational Modeling of Catheter-Based Reversible Cardiac Electroporation for Gene Delivery and Atrial Fibrillation Treatment
  Benjamin Sanchez, Albert Fabregas, Aleksei Mikhailov, David Johnson, Rishi Arora
• 14:15–14:30 O34 Improving Gene Electrotransfer with Gold Nanoparticles: Efficacy Comparison of Microsecond and Nanosecond Electric Fields
  Veronika Malyško, Eivina Radzevičiūtė-Valčiukė, Anna Szewczyk, Barbora Lekešytė, Paulina Malakauskaitė, Eglė Mickevičiūtė-Zinkuvienė, Augustinas Želvys, Natalija German, Julita Kulbacka, Vitalij Novickij
• 14:30–14:45 O35 Asymmetric Biphasic Pulsing for use in Gene Electrotransfer to Skin and Skeletal Muscle with Reduced Muscle Activation
  Alex Otten, Michael Francis, Richard Heller, Mark J. Jaroszeski, Nadezhda Korostyleva, Jacob Hensley, Tina Gagliardo, Lina Sabkhangulova, Anna Bulysheva
• 14:45–15:00 O36 Measurement of Electrical Conductivity in Biological Tissues Under High-Field Pulsed Conditions with Controlled Field Homogeneity
  Martin Hemzal, Dalibor Cervinka, Veronika Novotna

• 13:30–13:45 O19 Enhanced antitumor effects of electroporation combined with chemotherapy and PD-1 checkpoint inhibition in a bilateral 4T1 murine model
  Greta Chlebopaševienė, Diana Šiurnaitė, Paulius Ruzgys, Saulius Šatkauskas, Rasa Jančiauskienė, Erika Korobeinikova
• 13:45–14:00 O20 Skin dendritic cells mobilization upon electrochemotherapy: a dynamic analysis by 2-photons fluorescence microscopy.
  Elisabeth Bellard, Manon Gimbert, Simona Kranjc Brezar, Maja Cemazar, Marie-Pierre Rols, Bostjan Markelc, Muriel Golzio
• 14:00–14:15 O21 Deciphering tissue response and immune activation after Irreversible electroporation in Barrett’s Oesophagus.
  Lorraine Smith, Cian Gargan, Sam Cahill, Matthew McElheron, Rebecca Lyons, Aisling Uí Mhaonaigh, Kate Gorman, Lisa Travers, Fiona O'Conell, Cillian O'Donovan, Marie O'Brien, Jessie Eliott, Ravi Narayanasamy, Dermot O'Toole, Claire Donohue, Noel Donlon, David Prichard, Joanne Lysaght, Declan Soden, Jacintha O'Sullivan
• 14:15–14:30 O22 Novel Combination of Irreversible Electroporation and Allogenic Chimeric Antigen Receptor T-Cell Therapy Synergizes Therapeutic Outcomes in a Preclinical Pancreatic Cancer Mouse Model
  Julio Arroyo, Edward Jacobs, Sam Salemizadeh Parizi, Wei Guo, Yong Lu, Rafael Davalos
• 14:30–14:45 O23 Electroporation Improvement Using Conductive Nanoparticles: Successful Applications and Inconsistencies
  Eivina Radzevičiūtė-Valčiukė, Eglė Mickevičiūtė-Zinkuvienė, Augustinas Želvys, Barbora Lekešytė, Paulina Malakauskaitė, Veronika Malyško, Natalija German, Rokas Žalnėravičius, Vytautas Kašėta, Julita Kulbacka, Vitalij Novickij
• 14:45–15:00 O24 DNA Vaccines in the Era of Precision Medicine and Pandemic Preparedness: the use of electroporation as delivery system
  Luigi Aurisicchio

• 13:30–13:45 O25 Characterization of Myocardial Tissue Dielectric Properties After Pulsed Field Ablation Using Multi-Frequency Bioimpedance Spectroscopy
  Álvaro Borachok, Gerard Amorós-Figueras, Sergi Casabella-Ramon, Zoraida Moreno-Weidmann, Álvaro Borachok, Javier Rosell-Ferrer, Antoni Ivorra, Jose M. Guerra
• 13:45–14:00 O26 Titratable Deep Ventricular Lesions Using a Focal Force-Sensing Pulsed Field Ablation Catheter
  Lakshya Mittal, Jacob S. Koruth, Ammar M. Killu, Justo J. Torres-Rodriguez, Chris Lafean, Trenton Callaway, Hannah J. Loughlin, Catherine Pipenhagen, Jeffrey M. Fish
• 14:00–14:15 O27 Electrocardiology modeling after pulsed-field and radiofrequency ablations in ventricular tachycardia
  Simone Nati Poltri, Guido Caluori, Pierre Jaïs, Clair Poignard, Annabelle Collin
• 14:15–14:30 O28 Combined Pulsed Field Ablation and Localized Ethanol Delivery Increase Ventricular Lesion Depth: An Acute In Vivo Porcine Study
  Hanxu Niu, Ikram Haq, Jason Tri, Samuel J. Asirvatham, Duy Nguyen
• 14:30–14:45 O29 Thrombogenic Effects of Pulsed Electric Fields in Cardiac Ablation: Mechanistic Insights and Therapeutic Perspectives for Safer Procedures
  Pamela Sowa, Anne-Katrin Rohlfing, Zoi Laspa, Aleksander Kielbik, Vitalij Novickij, Oliver Borst, Meinrad Gawaz
• 14:45–15:00 O30 Physically-based numerical model for pulsed field ablation
  Simon Bihoreau, Michael Leguèbe, Guido Caluori, Pierre Jaïs, Clair Poignard, Annabelle Collin

• Coquina Salon A
• P1 B-01 Electroporation as an Alternative to Ultrafiltration for the Selective Recovery of Bioactive Yeast Peptides
  Javier Marín-Sánchez, Alejandro Berzosa, Ignacio Álvarez, Juan Manuel Martínez, Javier Raso, Ana Cristina Sánchez-Gimeno
• P2 B-02 Nisin as a Novel Membrane-Active Adjuvant to Overcome Bipolar Cancellation in Nanosecond Electrochemotherapy
  Veronika Malyško, Ausra Nemeikaite Ceniene, Olga Michel, Arnoldas Morozas, Zofia Łapińska, Eglė Mickevičiūtė-Zinkuvienė, Paulina Malakauskaitė, Augustinas Želvys, Justinas Ivaska, Julita Kulbacka, Vitalij Novickij
• P3 B-03 Overcoming the immune desert: spatial determinants of abscopal effect following electrochemotherapy in metastatic melanoma
  Maja Cemazar, Tina Kos, Gorana Gašljević, Iva Santek, Maša Omerzel, Gregor Sersa, Barbara Perić
• P4 B-04 The influence of microplastic particles (MPs) and electroporation (EP) on the type of cell death in the breast cancer cells.
  Katarzyna Bieżuńska-Kusiak, Nina Rembiałkowska, Anna Szewczyk, Julita Kulbacka, Anna Choromańska, Wojciech Szlasa
• P5 B-05 Exploring the Synergistic Potential of a Novel Anticancer Treatment by Using a Trimodal Combination of Ionizing Radiation, Electroporation, and Cisplatin
  Paulius Ruzgys, Simona Gelažunaitė, Greta Chlebopaševienė, Erika Korobeinikova, Saulius Šatkauskas
• P6 B-06 Preclinical Evaluation of DEBIO 1143 in Combination with Electrochemotherapy in Oral Squamous Cell Carcinoma
  Katarina Znidar, Veronika Skrjanc, Maja Cemazar, Ursa Lampreht Tratar, Jaka Vrevc Zlajpah, Tanja Jesenko
• P7 B-07 Feasibility of electroporation-based electrochemotherapy for hepatic and pancreatic tumors in dogs and cats
  Sergio S. Salgado, Nandy Ken Candela, Andrea Bazan, Mitzi Westreicher, David Mestanza, Micaela Vizquerra
• P8 B-08 Safety and Efficacy of Bleomycin Electrosclerotherapy in Low‑Flow Vascular Malformations Resistant to Sclerotherapy: A Prospective Cohort Comparing Straight and Cluster Electrodes
  Genti Xhepa, Annouk Anne Bisdorff- Bresson, Baptiste Bonnet, Benoit Faucon, Vittorio Civelli, Paul Beunon, Alexis Ricoeur, Lluis M. Mir, Lambros Tselikas, Frédéric Deschamps
• P9 B-09 Electrochemotherapy in the treatment of chronic suppurative benign skin conditions: Update on the St George’s Hospital experience
  Joy Odili
• P10 B-10 Electroporation-induced stress modulates ADAR expression and RNA editing dynamics in colorectal cancer cells
  Giorgia Notartomaso, Francesco De Leonardis, Domenico Alessandro Silvestris, Miriam Minervini, Elisabetta Notario, Marinella Marzano, Ermes Filomena, Chiara Lasconi, Paola Spadavecchia, Emanuela Signori, Anna Maria D'Erchia, Graziano Pesole, Ernesto Picardi, Mariangela De Robertis
• P11 B-11 Recombinant Gelonin for Electroporation-Based Intracellular Delivery In-Vitro
  Eleni Zivla, Giedre Silkuniene, Andrei G. Pakhomov, Mantas Silkunas, Olga N. Pakhomova
• P12 B-12 Emodin Modulates Cellular Response to Electrochemotherapy using High-Frequency Nanosecond Pulsed Electric Fields via Redox-Dependent Mechanisms
  Nina Rembiałkowska, Martyna Trznadel, Zofia Łapińska, Anna Szewczyk, Wojciech Szlasa, Katarzyna Bieżuńska-Kusiak, Vitalij Novickij, Eivina Radzevičiūtė-Valčiukė, Julita Kulbacka
• P13 B-13 Electroporation-Based Engineering of EGFR-Targeted Exosome-Producing 293T Cells for Precision Lung Cancer Therapy
  Anna Choromańska, Wojciech Szlasa, Agnieszka Gajewska-Naryniecka, Julita Kulbacka
• P14 B-14 Engineering recombinant gelonin for improved tissue penetration in pulsed electric field-targeted ablation
  Olga N. Pakhomova, Eleni Zivla, Giedre Silkuniene, Mantas Silkunas, Iurii Semenov, Andrei G. Pakhomov
• P15 B-15 Cytotoxic effect of BOLD-100 in combination with electroporation in human pancreatic cancer cell lines PANC-1 and BxPC-3
  Barbara Lisec, Nejc Koc, Maja Cemazar, Iztok Turel, Tanja Jesenko
• P16 B-16 Development of a Specific Electrode for the Treatment of Recurrent Tracheal Stenosis by Electrochemotherapy
  Sofia Pierri, Gian Franco Ramallo, Sebastian Michinski, Felipe H. Maglietti
• P17 B-17 A Novel Commercial Research Platform Enables Selective Lysis and Subpopulation Enrichment Across Heterogeneous Biological Models
  Terje Wimberger, Sandra Reischl, Julia Dolezel, Dorothea Pittrich
• P18 B-18 Investigation of cancer knowledge and awareness of environmental exposure in two districts of Istanbul
  Ayşe Emel Önal, Gülsüm Nurhan İnce, Meryem Merve Ören Çelik, Mustafa Oğuz Altuğ
• P19 B-19 Dose-Dependent Early Mitochondrial Responses of Breast Cancer Cells to Nanosecond Pulsed Electric Fields
  Augustine Ofeh, Lifang Yang, Siqi Guo
• P20 B-20 Finite Element Optimization of a Minimally Invasive Tetrapolar Electrode for Bone Cavities
  Guilherme Brasil Pintarelli, Lucas Bertinetti Lopes, Clara Teresa de Souza Ramos, Jéssica Rodrigues da Silva, Jânio Anselmo, Marcelo Monte Mór Rangel, Daniela Ota Hisayasu Suzuki
• P21 B-21 Extracting Protein from Chlorella Vulgaris Efficiently Using Pulsed Electric Discharge Shock Waves
  Seyedmasih Hosseini, Shirin Khakpour, Shintaro Ida, Hamid Hosano
• P22 B-22 Effect of Pause Time Between Electroporation and Electrolysis on Cumulative Ablative Effect in a Potato Model
  Sarah Gold, Erika Pinto, Rachel St. Germain, Lydia Abbazia, Mary Phillips Ho
• P23 B-23 Effect of impulsive pressure on Saccharomyces cerevisiae for the non-distractive protein extraction
  Kazuma Hata, Kazuyuki Saito, Sunao Katsuki, Satoshi Sakamoto, Katsuyuki Amino, Masato Shigematsu
• P24 B-24 Ns-PEFs Permeabilize the Entire Potato Tissue Region
  Haruki Ogami, Sunao Katsuki, Ikuya Koura
• P25 B-25 Extraction of Large Proteins from Yeast Using Intense Pulsed Electric Fields Combined with Pressurized Atomization
  Masamori Higuchi, Kazuma Hata, Haruki Ogami, Ikuya Koura, Masayuki Saitoh, Sunao Katsuki
• P26 B-26 Pulsed electric field assisted extraction of soybean seed protein: Optimization and structural characterization
  Tharun Kumar Shanmugam, Rivka Cahan
• P27 B-27 Temperature Dependent Dielectric Properties of Bacteria for Effective PEF Pasteurization
  Hajime Oniki, Miku Murakami, Sunao Katsuki, Kazuhiro Isobe, Taiga Kajiwara, Shoko Ishikawa, Ryo Sasahara, Naoya Masuda, Yoshiharu Shimizu, Jiro Kurihara
• P28 B-28 BIOAQUAPEF: A New Cross-Border POCTEFA Project (Spain-France) for the Sustainable Biorefinery of Aquatic Biomass Using Pulsed Electric Fields
  Juan Manuel Martínez, Javier Raso
• P29 B-29 Effects of Calcium Electrontransfer and Gold Nanoparticles on Yeast and Bacteria Inactivation
  Jurgita Švedienė, Gediminas Staigvila, Algimantas Paškevičius, Svetlana Markovskaja, Veronika Malyško, Vitalij Novickij, Jurij Novickij
• P30 B-30 Enhancing Microbial Safety of Bovine Colostrum Using Pulsed Electric Field Processing
  Indrawati Oey, Jessie King, Maddie Hardie-Boys, Daniel Pletzer, Phil Bremer
• Coquina Salon F
• P31 B-31 Monitoring Intracellular Calcium Under Long-Term Moderate Electric Field Exposure
  Masoud Hosseinidoost, Leslie Vallet, Aracelys García Moreno, Burak Altun, Ferran Camps Carre, Antoni Ivorra
• P32 B-32 Modulation of Nanosecond Pulsed Electric Field–Based Electrochemotherapy by Estrogen Alternatives
  Zofia Łapińska, Jolanta Harasiuk, Nina Rembiałkowska, Anna Szewczyk, Olga Michel, Dagmara Baczyńska, Wojciech Szlasa, Vitalij Novickij, Julita Kulbacka
• P33 B-33 Air–liquid interface distorts electric field distribution and electroporation of adherent cells
  Yorgos Kougkolos, Yara Abidine, Manuel Marcoux, Muriel Golzio, Marie-Pierre Rols, Maxime Berg
• P34 B-34 Finite Element Method Analysis of Nanoparticle-Enhanced Electroporation
  Caterina Bernardini, Elisabetta Sieni, Patrizia Lamberti, Maria Evelina Mognaschi, Paolo Di Barba, Fabrizio Dughiero, Vincenzo Tucci
• P36 B-36 From Electroporation Hypothesis to Thermal Mechanism in TTFields-Treated U87 Cells
  Burak Altun, Aracelys García Moreno, Ferran Camps Carre, Leslie Vallet, Masoud Hosseinidoost, Antoni Ivorra
• P37 B-37 Spatial Magnetic Field Simulation Research on the Application of Pulsed Electromagnetic Field Technology in Microcirculation
  Kaixuan Xu, Jizhuang Zhang
• P38 B-38 Previously Unrecognized Nuclear Vulnerabilities to nsPEF: INM Protein Complex Disruption and Chromosome-End Instability.
  Michael Stacey, Gavin Vasquez
• P39 B-39 Multi-Omics Profiling Reveals a Global Collapse of Cellular Homeostasis and Immunogenic Reprogramming Induced by Nanosecond Pulsed Electric Fields (nsPEFs)
  Siqi Guo, Lifang Yang
• P40 B-40 Scanning electrochemical microscopy for localized assessment of electroporation-induced redox responses
  Inga Morkvenaite, Antanas Zinovicius, Daiva Bironaite, Rokas Miksiunas, Laisvidas Striska, Tomas Mockaitis, Evaldas Balciunas, Rokas Bagdonas, Arunas Ramanavicius
• P41 B-41 Musa acuminata as a Rapid Phantom for Rod-Electrode Evaluation
  Daniella de Lourdes Luna Santana de Andrade, Clara Teresa de Souza Ramos, Jéssica Rodrigues da Silva, Jânio Anselmo, Guilherme Brasil Pintarelli, Daniela Ota Hisayasu Suzuki
• P42 B-43 Comprehensive thermal evaluation of electroporation experiments in tissue-mimicking 3d collagen matrix
  Veronika Malyško, Vitalij Novickij, Damijan Miklavčič, Bor Kos
• P43 B-44 Automated High Throughput Lab-on-Chip Workflow for Improving Electrotransfection in Adherent Mammalian Cells
  Paul Fong, Fan Yuan
• P44 B-45 Numerical Modeling of Pulsed Field Ablation Probe Geometries for Transmural Ventricular Lesion Formation
  Nyah Ebanks, Pedro Paulo Santos, Rafael Davalos
• P45 B-46 The Phase characteristics of the biological effects of pulsed electric fields on tissues ablation
  Weiwei Huang, Jiali Bao
• P46 B-47 Frequency-Dependent Electric Field Thresholds in Irreversible Electroporation: A Numerical Study
  Ting Shu, Antoni Ivorra
• P47 B-48 Computational Analysis of Electroporation in Peripheral Nerves: Nodal Vulnerability and Fiber-Type Dependence
  Berta Mateu-Yus, Antoni Ivorra
• P48 B-49 ERASE the Limits: Optimised Electroporation Pulse Structuring for Non-Thermal, Apoptosis-Dominant Tumour Ablation
  Ciara Nulty
• P49 B-50 Multiphysics Modeling of Epicardial Pulsed Field Ablation for Cardiac Arrhythmias: Impact of Coronary Artery and Stent Proximity
  Francisco Estevez-Labori, Maite Izquierdo, Ken Coffey, Barry O’Brien, Ana González-Suárez
• P50 B-51 Linking Computed Electric Dose Maps and Early Post-Operative MRI After Irreversible Electroporation
  Olivier Sutter, Baudouin Denis de Senneville, Olivier Seror, Clair Poignard
• P51 B-52 Electroporation-based delivery of trans-amplifying mRNAs for cancer gene therapy
  Jaka Vrevc Žlajpah, Urška Kamenšek, Ursa Lampreht Tratar, Maja Cemazar, Gregor Sersa, Katja Uršič Valentinuzzi
• P52 B-53 Dense Encapsulated Tissue Mimics for High-Throughput Gene Electrotransfer Evaluation
  Arianna Escalona, Julio Arroyo, Rafael Davalos
• P53 B-54 Modernizing Coley’s concept: engineering secretable toxin-encoding plasmids for in situ tumor vaccination via gene electrotransfer
  Urška Kamenšek, Maja Cvetanoska, Maja Cemazar, Gregor Sersa
• P54 B-55 Interaction-Driven Assembly of Protein–Hydrophobic Molecule Systems: Implications for Bioelectric-Assisted Delivery
  Shirin Khakpour, Zahra Moosavi-Nejad, Nushin Hosano, Shintaro Ida, Hamid Hosano
• P55 B-56 Toward an Optimal Dose–Response in Electroporation-Based Tumor Treatments: Pulse Number and Thermal Damage Trade-Offs
  Isaac Rodríguez, Ezequiel Goldberg, Nahuel Olaiz, Felipe H. Maglietti, Cecilia Suárez, Alejandro Soba, Guillermo R. Marshall
• P56 B-57 Electroporation-based evaluation of macrophage extracellular trap formation using micro- and nanosecond electric pulses in tumor-related in vitro models
  Anna Szewczyk, Katarzyna Bieżuńska-Kusiak, Julita Kulbacka
• P57 B-58 Bleomycin electrosclerotherapy of slow flow vascular malformations: The Ljubljana experience
  Gregor Sersa, Miha Stabuc, Dimitrij Kuhelj, Masa Omerzel, Barbara Lisec, Tine Arnez, Orijana Velikonja, Maja Cemazar
• P58 B-59 Comparison of Manual and Automated Plasmid Delivery for Electroporation-Mediated Gene Therapy.
  Hunter Fitzpatrick, Pedro Rihs, Mike Sano
• P35 Utilization of Pulsed Field Ablation to Treat Orthotopic Human Pancreatic Tumors in a Novel Genetically Modified Porcine Model
  Manali Powar, Edward Jacobs, Tamalika Paul, Levente Gal, Mackenzie Woolls, Carley Elliott, Cassandra Poole, Katie Orr, Christopher Byron, Sherrie Clark- Deen, Sheryl Coutermarsh-Ott, Kiho Lee, Rafael Davalos, Irving Coy Allen

• 16:30–16:45 O41 DISSECTING CELL DEATH PATHWAYS ACTIVATED BY ELECTROCHEMOTHERAPY
  Lara Snoj, Gregor Sersa, Amar Balihodzic, Simona Kranjc Brezar, Masa Omerzel, Tanja Jesenko, Michael Dengler, Maja Cemazar, Bostjan Markelc
• 16:45–17:00 O42 Dynamic model of tissue electropermeabilization with memory effects
  Irina Pettersson
• 17:00–17:15 O43 Beyond membrane permeabilization: Electroporation reshapes post-transcriptional regulation in colorectal cancer
  Mariangela De Robertis, Giorgia Notartomaso, Francesco De Leonardis, Domenico Alessandro Silvestris, Ermes Filomena, Miriam Minervini, Elisabetta Notario, Marinella Marzano, Chiara Lasconi, Paola Spadavecchia, Emanuela Signori, Anna Maria D'Erchia, Graziano Pesole, Ernesto Picardi
• 17:15–17:30 O44 Electrochemotherapy Combined with Immunotherapy: From Local Tumor Control to Systemic Antitumor Responses
  Maja Cemazar, Ursa Lampreht Tratar, Katja Uršič Valentinuzzi, Simona Kranjc Brezar, Maša Omerzel, Tanja Jesenko, Barbara Lisec, Bostjan Markelc, Nataša Tozon, Urška Kamenšek, Barbara Perić, Gregor Sersa

• 16:30–16:45 O45 Advance in pulsed electric fields (PEF)-based electric pasteurization in Food
  Lang-Hong Wang, Xinan Zeng
• 16:45–17:00 O46 New opportunity of using pulsed electric field (PEF) technology to produce texture-modified chickpea flour-based gels for people with dysphagia
  Federico Drudi, Indrawati Oey, Sze Ying Leong, Jessie King, Kevin Sutton, Silvia Tappi, Urszula Tylewicz
• 17:00–17:15 O47 Simultaneous pulsed electric field and ultrasound intensify beetroot tissue disintegration and antioxidant release through synchronization-driven electroacoustic destabilization
  Feiyue Xu, Chaojun Wang, Jialin Ma, Jing Zhuo, Xinan Zeng
• 17:15–17:30 O48 Application of pulsed electric field technology to improve dairy powder production
  Owen McDougal

• 16:30–16:45 O37 Patient-Specific Electric Field Simulation in Spinal Metastasis Electrochemotherapy
  Paul Beunon, Baptiste Bonnet, Lambros Tselikas, Paul Janvier, Genti Xhepa, Olivier Sutter, Baudouin Denis de Senneville, Luc Lafitte, Olivier Seror, Lluis M. Mir, Frédéric Deschamps, Clair Poignard
• 16:45–17:00 O38 High-Frequency Biphasic Electroporation Enables Tunable, Multi-Fold Enhancement of Tumor Biopsy Yield with Spatial Control
  Poompavai Sadasivam, Shengwei Wu, Govind Srimathveeravalli
• 17:00–17:15 O39 Preclinical modeling and pharmacokinetic insights for improved electrochemotherapy in osteosarcoma
  Sasa Kupcic, Maja Cemazar, Urška Kamenšek, Ursa Lampreht Tratar
• 17:15–17:30 O40 Establishment of Endothelial PIK3CA-H1047R Preclinical Models for the Study of Vascular Malformations and Bleomycin Electrosclerotherapy
  Barbara Lisec, Bostjan Markelc, Sara Čačič, Tanja Jesenko, Masa Omerzel, Andrej Coer, Maja Cemazar, Gregor Sersa

• 08:30–09:00 PT3 Multimodal Microfluidic Cell Engineering
  Abraham Lee
• 09:00–09:30 PT4 From irreversible electroporation to pulsed field ablation - The advent and evolution of electroporation in cardiac electrophysiology
  Suraj Kapa
• 09:30–10:00 PT5 Intratumoral Plasmid IL-12 Electro-Gene-Transfer and PD1 Blockade as Neoadjuvant Immunotherapy for Patients with Operable, Locoregionally Advanced Melanoma
  Ahmad Tarhini, Mohammad Ali Khaksar, Vernon Sondak

• 10:30–10:45 O49 Direct and indirect effects of electric fields on protein systems
  Michal Cifra, Saurabh Kumar Pandey, Tomáš Zakar, Michaela Poplová, Daniela G. Blanco Campoy, Jiří Průša, Daniel Havelka, Paolo Marracino, Micaela Liberti, Francesca Apollonio, Niall J. English, Carl Caleman, Erik G. Marklund
• 10:45–11:00 O50 ANNEXIN-A4 DRIVEN RAPID PHOSPHOLIPID MEMBRANE REPAIR DYNAMICS REVEAL TRANSITION FROM REVERSIBLE TO IRREVERSIBLE ELECTROPORATION
  Baltramiejus Jakštys, Haroldas Sakavičius, Saulius Šatkauskas
• 11:00–11:15 O51 Effects of MV class Pulsed Electric Fields on proteins
  NeNe Yoshioka, Sho Mawatari, Sunao Katsuki
• 11:15–11:30 O52 The Effect of Faradaic Charge-Transfer Reactions on Cell Permeabilization and Viability During Electroporation
  Dominik Martinů, Urša Primožič, Alenka Maček-Lebar, Samo Mahnič, Martin Hemzal, Lea Rems, Eric D. Głowacki
• 11:30–11:45 O53 Impact of vascular lock on blood flow distribution and vessel electropermeabilisation under pulsed electric fields: experiments and simulations
  Elisabeth Bellard, Nolwen Kermoal, Sandrine Pelofy, Marie-Pierre Rols, Justin Teissie, Maxime Berg, Muriel Golzio
• 11:45–12:00 O54 PEF sequence architecture determines the electrochemical effects on tubulin
  Tomáš Zakar, Michaela Poplová, Kateřina Červinková, Daniel Havelka, Ondřej Kučera, Viliam Kolivoška, Michal Cifra

• 10:30–10:45 O55 Nanosecond Pulsed Field Ablation for the treatment of atrial fibrillation and thyroid nodules
  Richard Nuccitelli
• 10:45–11:00 O56 Electroporation-Mediated Gelonin Cytotoxicity Triggers Delayed Apoptotic Cell Death with ATP Release
  Giedre Silkuniene, Mantas Silkunas, Eleni Zivla, Andrei G. Pakhomov, Olga N. Pakhomova
• 11:00–11:15 O57 Nanosecond pulsed electric fields induce immunogenic cell death and activate antigen-presenting cells in penile cancer
  Aleksander Kielbik, Ilona Hagelstein, Pamela Sowa, Maria Luisa Barcena, Vitalij Novickij, Igor Tsaur
• 11:15–11:30 O58 Effects of Nisin and Ultra-fast Nanosecond Electric Field Pulses on Cancer Cells and Mitochondria
  Paulina Malakauskaitė, Vytautas Kašėta, Vitalij Novickij
• 11:30–11:45 O59 Effects of Nisin Z on Cell Sensitivity to Electroporation using Dielectrophoretic Characterization and 3D Spheroids
  Olga Michel, Barbora Lekešytė, Zofia Łapińska, Ioan Cristian Tivig, Florela Gherghinoiu, Mihaela G. Moisescu, Julita Kulbacka, Vitalij Novickij
• 11:45–12:00 O60 A New Approach for Endoscopic Electrochemotherapy of Recurrent Upper Gastrointestinal Tumors: Literature Review and Technical Feasibility
  Seyed Mojtaba Yazdanparast, Habibollah Mahmoodzadeh, Seyed Rouhollah Miri, Mohamad Abdolahad

• 10:30–10:45 O61 Electrochemotherapy for cutaneous Merkel Cell Carcinoma: A European register-based cohort study from the International Network for Sharing Practices of electrochemotherapy (InspECT)
  Joy Odili
• 10:45–11:00 O62 Electrochemotherapy combined with tumor irradiation either before or after tumor irradiation; feasibility, safety and effectiveness analysis from INSPECT database
  Giulia Bertino, Julie Gehl, Erika Kis, Hadrian Nassabi, Michele Pio Grieco, Giuseppe Riva, Matteo Mascherini, Francesca De Terlizzi, Gregor Sersa
• 11:00–11:15 O63 From Approval to Acceptance: The Clinical Journey of ECT in Argentina
  Felipe H. Maglietti, Jesica Miranda, Matías N. Tellado, Marina Riveiro, Sebastian D. Michinski, Guillermo R. Marshall
• 11:15–11:30 O64 Electroporation-Enhanced Delivery of Gelonin in ESCC Xenografts: Histological Evidence of Tumor Cell Damage
  Pavel A. Solopov, Olga N. Pakhomova, Alayna Enos, Mantas Silkunas, Giedre Silkuniene, Eleni Zivla, Andrei G. Pakhomov
• 11:30–11:45 O65 Percutaneous Electrochemotherapy for soft tissue tumors: Local Tumor Control, Pain Palliation, and Safety Outcomes
  Genti Xhepa, Baptiste Bonnet, Paul Beunon, Paul Janvier, Matthieu Garnier, Pierre Faerber, Jean-Philippe Rives, Mathieu Faron, Benjamin Besse, Lluis M. Mir, Lambros Tselikas
• 11:45–12:00 O66 Electrochemotherapy in the treatment of extrahepatic bile duct tumors – a pilot study
  Aurel Ottlakan, Tibor Geczi, Gabor Vass, Gyorgy Lazar, Judit Olah, Erika Kis

• 13:30–13:45 O73 Investigating the Frequency-Dependent Effects of Picosecond Pulses on Calcium Mobilization in CHO-K1 Cells
  Eucharia Ochigbo, Shu Xiao, Iurii Semenov, Stephen J. Beebe
• 13:45–14:00 O74 RF-Based Nanosecond Electric Pulses for In Vivo-Compatible Neuromodulation: A Digitally Synthesized Composite Chirp Approach
  Kyung Eun You, Jihwan Yoon
• 14:00–14:15 O75 An Impedance-Matched High-Voltage Microfluidic Device for Sub-ns PEF and Electroporation Exploration
  Lionel Michard, Jules Pimont, Claire Dalmay, Philippe Leveque, Delia Arnaud-Cormos
• 14:15–14:30 O76 Preclinical Study of Irreversible Electroporation Ablation System for Pulmonary Nodule Ablation
  Chao Chen
• 14:30–14:45 O77 EMF Impedancemetry based delivery systems for the study of ion channels exposed to nanosecond pulses
  Nina Andrée Van, Lionel Michard, Nina Reitano Ferber, Gilles N’Kaoua, Florence Poulletier de Gannes, Delia Arnaud-Cormos, Yann Percherancier, Isabelle Lagroye, Philippe Leveque, Noelle Lewis, Rosa Orlacchio
• 14:45–15:00 O78 Hybrid Microwave-PEF platform for sensing PEF-induced electrochemical effects
  Petr Kůrka, Jack McClelland, Viliam Kolivoška, Mohammad Zarifi, Michal Cifra, Daniel Havelka

• 13:30–13:45 O79 Hemolysis in Pulsed Field Ablation: A Multiscale Numerical Framework
  Melinda Quan, Quim Castellvi
• 13:45–14:00 O80 A MeshGraphNet Surrogate for Stationary Electric Field Simulation and Optimization of Pulsed Field Ablation
  Pedro Paulo Santos, Rafael Davalos
• 14:00–14:15 O81 Surgical Treatment of Cardiac Arrhythmias: Chronic Preclinical Results and Epicardial Ablation
  Christian W. Zemlin
• 14:15–14:30 O82 Pulsed Field Ablation for Symptomatic Junctional Arrhythmias
  Ikram Haq, Jason Tri, Ammar Killu, Alan Sugrue, Nicholas Tan
• 14:30–14:45 O83 Initial Single Center US Experience with Pulse Field Ablation
  Amalia Welle, Shivaraj Patil, Samuel J. Asirvatham, Adhishek Deshmukh, Christopher V DeSimone, Ammar Killu, Gurukripa Kowlgi, Suraj Kapa
• 14:45–15:00 O84 Comparative Efficacy and Procedural Efficiency of Pulsed Field Ablation Systems (Centauri, Farapulse) versus Conventional Radiofrequency Ablation in Atrial Fibrillation
  Martin Pesl, Filip Soucek, Katarina Dolezalova, Vojtech Sebetovsky, Katarina Melajova, Zdenek Starek

• 13:30–13:45 O67 A Fast and Clinically Compatible Numerical Workflow for HCC Electroporation Ablation
  Olivier Sutter, Baudouin Denis de Senneville, Olivier Seror, Clair Poignard
• 13:45–14:00 O68 Electrochemotherapy for sacral metastases: a retrospective study on efficacy, safety, and pain palliation
  Genti Xhepa, Emilie Devars, Baptiste Bonnet, Paul Beunon, Paul Janvier, Matthieu Garnier, Pierre Faerber, Jean-Philippe Rives, Lambros Tselikas, Frédéric Deschamps
• 14:00–14:15 O69 Novel Synergistic Pulsed Electric Fields: Opening a New Pathway for Efficient Tumor Ablation under Local Anesthesia
  Hongmei Liu, Shoulong Dong, Chenguo Yao
• 14:15–14:30 O70 Hidradenitis suppurativa treatment with ECT – Pilot Study Protocol
  Erika Kis, Gabor Vass, Zsuzsanna Besenyi, Doniz Degovics
• 14:30–14:45 O71 Electrochemotherapy in the Management of Advanced Head and Neck Tumors: Initial Clinical Experience from a Single Center
  Gabor Vass, Aurel Ottlakan, Erika Kis
• 14:45–15:00 O72 A new transurethral electrode for electroporation in the urinary bladder – preclinical evaluation, clinical adaptation, and perspectives
  Rose Bryde Laursen, Zaid S. Salameh, Kailee David, Trine Hallager, Pernille Holst, Thomas Eriksen, Rafael Davalos, Julie Gehl, Juan Luis Vásquez

• 16:30–16:45 O89 Optimising Circular RNA Therapeutics for Cancer Immune Modulation
  Megan McAuley, Ciara Nulty, Declan Soden, Jacintha O'Sullivan, Vincent Kelly
• 16:45–17:00 O90 Effective Application of Immunotherapy for the Treatment of Melanoma Using Pulse Field Delivery
  Richard Heller, Loree Heller, Jody Synowiec, Julie Singh, Alex Otten, Mark J. Jaroszeski
• 17:00–17:15 O91 Study of the activation of the anti-tumor immune response following electrochemotherapy combined with the electrotransfer of plasmids encoding immune checkpoint inhibitors used in immunotherapy
  Manon Gimbert, Elisabeth Bellard, Simona Kranjc Brezar, Emmanuelle Naser, Maya Imbrechts, Tanja Jesenko, Masa Omerzel, Maja Cemazar, Marie-Pierre Rols, Nick Geukens, Kevin Hollevoet, Bostjan Markelc, Muriel Golzio
• 17:15–17:30 O92 Gene electrotransfer of mesenchymal stem cells for cancer immunotherapy: from interleukin 12 delivery to multimodal armament
  Urška Kamenšek, Urban Švajger, Tim Božič, Maja Cemazar

• 16:30–16:45 O93 An isoenergetic comparison of pulsed electric field (PEF) and ultrasound (US) treatments for extraction of valuable compounds from Agaricus bisporus mushrooms
  Federico Drudi, Maria Alessia Schouten, Pietro Rocculi, Santina Romani, Urszula Tylewicz
• 16:45–17:00 O94 Pulsed electric field as a sustainable strategy for obtaining a pectin-rich extract from overripe papaya (Carica papaya L. cv. Maradol) powder
  Giulliana Torrano, Flavia Almeida, Bruno Carciofi, Carmen Tadini
• 17:00–17:15 O95 Pulsed Electric Field stimulate the production of nitric oxide in fruits: Mechanism Elucidation and Preservation Efficacy
  Rui Wang, Ben Yu, Qi-Qing Liang, Ai Xu, Xinan Zeng
• 17:15–17:30 O96 Optimization of Pulsed Electric Field (PEF) Assisted Extraction of Basil Seed (Ocimum basilicum) Mucilage: Impact of Process Parameters on Yield and Functional Performance
  Arun Sharma, Gudaranakamma Vuyyuru, Malay Yadav, Aniket Mone

• 16:30–16:45 O85 Effects of 5G-modulated 26 GHz radiofrequency fields on oxidative stress mechanisms in human skin models
  Yorgos Kougkolos, Géraldine Alberola, Delia Arnaud-Cormos, Philippe Leveque, Muriel Golzio, Marie-Pierre Rols
• 16:45–17:00 O86 High frequency biphasic pulsed electric field treatment of endothelial cells produces mechano-mimetic outcomes with potential therapeutic applications
  Neeraj Raghuraman Rajagopalan, Cecilia Labbe, Stephanie Hallerman, Oliver Rose, Mrinmoy Nandi Bappa, Govind Srimathveeravalli
• 17:00–17:15 O87 Investigating the Spatiotemporal Dynamics of Electroporation-Induced Cell Death Mechanisms
  Kailee David, Edward Jacobs, Rafael Davalos
• 17:15–17:30 O88 Characteristics of extracellular DNA influence cell membrane damage induced by electrical short-circuiting through an aqueous droplet in dielectric oil
  Hikaru Hasegawa, Yoshino Tsurusaki, Rika Numano, Hirofumi Kurita

• 08:30–09:00 PT6 Bioresorbable Injectable In Situ–Formed Electroactive Device: From Brain Tumor Electroimmunotherapy to Regenerative Gene Delivery
  Roger Olsson
• 09:00–09:30 PT7 Engineering the Next Generation of Pulsed Electric Field Technologies: From Beverage Preservation to Sustainable Processing of Seeds and Spices
  Gulsun Akdemir Evrendilek
• 09:30–10:00 PT8 Mechanisms and Modulation of DNA Transport in Electroporated Cells: From Extracellular Space to the Nucleus
  Fan Yuan

• 10:30–10:45 O97 Lightning Rod Approach: Enhanced Cell Selective Low-Voltage Electroporation for In-Vivo Cell Manufacturing
  Vaishali Malik, Badal Singh, Sankaran Thayumanavan, Govind Srimathveeravalli
• 10:45–11:00 O98 Challenges and Opportunities in Electrotransfer for Nucleic Acid and Protein Delivery
  Pouyan E. Boukany
• 11:00–11:15 O99 Optimization of Non-Viral CRISPR/Cas9 Electrotransfer for Targeted Gene Therapy
  Ernestas Urbanskas, Raulas Krušauskas, Justinas Venckus, Aras Rafanavičius, Rūta Palepšienė, Baltramiejus Jakštys, Paulius Ruzgys, Saulius Šatkauskas
• 11:15–11:30 O100 Enhanced Ablation Depth of Pulsed Electric Fields via Pulse Width Compression: "Bullet" Pattern and Its Quantitative Analysis
  Shupeng Wang, Hongyu Kou, Runze Liang, He Xu, Chenguo Yao
• 11:30–11:45 O101 Electroporation-Driven Cas9 RNP Delivery into Hepatocytes Enables a Promising Cell Therapy for Severe Familial Hypercholesterolemia
  Menam Pokhrel, Renee Cottle, Abishek Dhungana
• 11:45–12:00 O102 Advancing Off-the-Shelf Cell Therapies: Cryopreservation of Electroporation-Mediated CRISPR-Cas9 Gene-Edited Primary Hepatocytes.
  Abishek Dhungana, Renee Cottle, Menam Pokhrel

• 10:30–10:45 O103 High-Frequency Electrochemotherapy: Optimized Protocols for Enhanced Efficacy and Improved Comfort—Validation from 3D Models to Animal Clinical Trials
  Alexia de Caro, Jean-Baptiste Leroy, David Sayag, Ilaria Marano, Elodie Lallemand, Marion Toussaint, Jelena Kolosnjaj-Tabi, Marie-Pierre Rols, Muriel Golzio
• 10:45–11:00 O104 Electroporation-based electrochemotherapy for canine acanthomatous ameloblastoma: Treatment response and long-term outcomes
  Sergio S. Salgado, Nandy Ken Candela, David Mestanza, Micaela Vizquerra, Andrea Bazan, Mitzi Westreicher
• 11:00–11:15 O105 Electrode Needle Degradation in Electrochemotherapy: Mechanisms, Effects, and Mitigation Strategies
  Maria Miranda, Antonella Cilio, Ana Campastri, Marina Riveiro, Victor Farricha, Sebastian Michinski, Felipe Magliett
• 11:15–11:30 O106 Bone-Targeted Electrochemotherapy for Feline Oral Squamous Cell Carcinoma Using a Single-Needle Electrode: A Three-Case Series
  Arbel Vigodny, Joseph A. Impellizeri
• 11:30–11:45 O107 Electroporation-based electrochemotherapy for rectal adenocarcinoma in dogs: Clinical outcomes from an expanded case series
  Sergio S. Salgado, Mitzi Westreicher, Micaela Vizquerra, David Mestanza, Nandy Ken Candela, Andrea Bazan
• 11:45–12:00 O108 Electroporation-based electrochemotherapy for canine perianal tumors: Treatment response and clinical performance
  Sergio S. Salgado, Micaela Vizquerra, Andrea Bazan, Mitzi Westreicher, David Mestanza, Nandy Ken Candela

• 10:30–10:45 O109 Intracellular ROS modulation in pancreatic cancer cells by pulsed plasma jets with variable pulse durations
  Chunqi Jiang, Edwin A. Oshin, Zobia Minhas, Jayani H. Edirisooriyage, Siqi Guo
• 10:45–11:00 O110 Low-Temperature Plasma: A Novel Strategy for Promoting Regenerative Repair of Chronic Refractory Wounds
  Jing Liu
• 11:00–11:15 O111 Comparing nanosecond pulsed plasma reactors for degradation of organic pollutants from water
  Zinat Nisha, Jayani H. Edirisooriyage, Eva Doty, Garrett Rodgers, Oliver Sedwick, Olga N. Pakhomova, Gary Schafran, Chunqi Jiang
• 11:15–11:30 O112 A Sustainable Approach for Fish Preservation: Shelf-Life Extension of Trout (Salmo trutta) Fillets Using Plasma-Activated Water (PAW) and PAW-Assisted Coatings
  Riccardo Marconi, Francesca Pompei, Luca Boldrini, Elettra Della Ceca, Gebremedhin Gebremariam Gebremical, Mattia Acito, Silvia Tappi, Cinzia Mannozzi, Sauro Vittori, Gianni Sagratini
• 11:30–11:45 O113 Nanosecond Pulsed Electric Field (nsPEF) Prime Pancreatic Cancer Cells for Cold Atmospheric Pressure Plasma Discharge (CAP)- Induced Apoptosis by Dismantling Adaptive Proteostasis
  Zobia Minhas, Edwin A. Oshin, Chunqi Jiang, Lifang Yang, Shanaya M. Haque, Siqi Guo
• 11:45–12:00 O114 Inhaled Cold Atmospheric Plasma Generated by Pulsed High-Voltage Discharge for Noninvasive Glioblastoma Treatment
  Runze Liang, He Xu, Ting Zhu, Kai Chen, Shoulong Dong, Chenguo Yao

• 13:30–13:45 O115 Linking Cell Surface Mechanics to Electroporation via a Microfluidic Aspiration Device
  Sophie de Boer, Pouyan E. Boukany
• 13:45–14:00 O116 Nanoneedle Electroporation Overcomes Endocytic Barriers to Genetic Engineering in Human Regulatory T Cells
  Ciro Chiappini
• 14:00–14:15 O117 Exploring Nanosecond Pulsed Electric field (nsPEF)-mediated electro-exocytosis of extracellular vesicles in immune cells
  Teresa Hooker, Stephen J. Beebe, Ruben Manuel Luciano Colunga Biancatelli
• 14:15–14:30 O118 Thermostable Nanoarchaeosome–Nanoneedle System for Ex Vivo CAR-T Cell Engineering in Breast Cancer.
  Swathi Sudhakar
• 14:30–14:45 O119 Silica-Coated Magnetic Nanoparticle Clusters Positioned Near the Plasma Membrane Locally Alter Electric Field Distribution and Improve Electroporation
  Elisabetta Sieni, Patrizia Lamberti, Vincenzo Tucci, Nicolas Mattei, Marie-Pierre Rols, Muriel Golzio, Slavko Kralj, Jelena Kolosnjaj-Tabi
• 14:45–15:00 O120 Microsecond-Scale Plasma Membrane Recovery after Electroporation as a Mechanism of Bipolar Cancellation
  Iurii Semenov, Giedre Silkuniene, Mantas Silkunas, Olga N. Pakhomova, Andrei G. Pakhomov

• 13:30–13:45 O121 Microsecond Pulsed Electric Fields as a Physical Senolytic Strategy for Selective Elimination of Senescent Cells
  Lei Li, Chenguo Yao
• 13:45–14:00 O122 Electrical Dynamics of Pulsed Field Ablation Cardiac Lesion Maturation: Using a Novel Monophasic Action Potential Characterization Tool
  James Villar-Mead, Gino DiMatteo, Paul A. Iaizzo
• 14:00–14:15 O123 A Bibliometric Study on IRE‑Triggered Immunogenic Cell Death and Its Mechanism in Synergistic Immunotherapy
  Fei Teng
• 14:15–14:30 O124 From Single Molecules to Clinical Response: A Multiscale Predictive Framework for Electroporation-Based Therapy in Locally Advanced Pancreatic Cancer
  Julita Kulbacka, Wojciech Szlasa, Julia Rudno-Rudzińska, Wojciech Kielan, Maciej Guziński, Ewelina Freilich, Jakub Segen, Zenon Chaczko, Agnieszka Gajewska-Naryniecka, Marek Kulbacki
• 14:30–14:45 O125 Burst Sine Wave Electroporation for High-Grade Glioma: Non-thermal Ablation in a Preclinical Rodent Study
  Sam Salemizadeh Parizi, Nyah Ebanks, John H. Rossmeisl, Rafael Davalos
• 14:45–15:00 O126 Electroporation-based Processes for Water Disinfection
  Xing Xie

• 13:30–13:45 O127 Balancing electroporation and protein extraction in PEF-treated Saccharomyces cerevisiae
  Javier Marín-Sánchez, Alejandro Berzosa, Juan Manuel Martínez, Ignacio Álvarez, Ana Cristina Sánchez-Gimeno, Javier Raso
• 13:45–14:00 O128 Harnessing Pulsed Electric Fields to Engineer Structure and Enhance Protein Digestibility in Plant-Based Composite Gels
  Indrawati Oey, I Gde Adi Suryawan Wangiyana, Federico Drudi, Nicholas Horlacher, Jessie King, Sze Ying Leong, Esther Kim, Kevin Sutton
• 14:00–14:15 O129 Importance of experimental setup and accurate process data reporting for investigation of electric field effects on cells and macromolecules
  Felix Schottroff, Henry Jaeger, Javier Raso
• 14:15–14:30 O130 Designing the Next Generation of Food Materials: Engineering Plant Protein Gels via Pulsed Electric Field
  Flavia Almeida, Nitin Nitin, Gail Bornhorst
• 14:30–14:45 O131 Pulsed electric field-assisted starch recovery from avocado seed: Structural tailoring and mechanistic insight into digestibility modulation
  Danela Silva-Ferrer, Dominique Larrea-Wachtendorff, Gipsy Tabilo-Munizaga, Mario Pérez-Won
• 14:45–15:00 O132 Development of Continuous Flow PEF Pasteurization System for Liquid Whole Egg
  Hayato Tajiri, Kenshin Hara, Sunao Katsuki, Naoya Masuda, Yoshiharu Shimizu, Kazuhiro Isobe, Taiga Kajiwara, Shoko Ishikawa, Ryo Sasahara

• 16:15–16:30 O138 Nanosecond electrostimulation of GCaMP6f-expressing chromaffin and satellite glial cells in murine adrenal slices
  Josette Zaklit, Ciara Viola, Lisha Yang, Jose Moreno Duran, Nicole M. Procacci, Normand Leblanc, Gale L. Craviso, Thomas W. Gould
• 16:30–16:45 O139 Rethinking phosphatidylserine externalization after electroporation: a vesicle-shedding mechanism
  Mantas Silkunas, Alayna Enos, Giedre Silkuniene, Andrei G. Pakhomov
• 16:45–17:00 O140 From ns to ms Pulses: Dynamics of Individual Electropores in Live Cells
  Mantas Silkunas, Giedre Silkuniene, Iurii Semenov, Olga N. Pakhomova, Andrei G. Pakhomov
• 17:00–17:15 O141 MILD Electropermeabilization as a Tool to Modulate Calcium Signaling
  Leslie Vallet, Masoud Hosseinidoost, Aracelys García Moreno, Burak Altun, Ferran Camps Carre, Antoni Ivorra
• 17:15–17:30 O142 Bioluminescence Resonance Energy Transfer (BRET)-based real-time monitoring of cellular responses to nanosecond pulsed electric fields for ion channel studies
  Nina Reitano Ferber, Nina Andrée Van, Patricia Nabos, Florence Poulletier de Gannes, Isabelle Lagroye, Philippe Leveque, Delia Arnaud-Cormos, Noelle Lewis, Rosa Orlacchio, Yann Percherancier

• 16:15–16:30 O143 Kinetics of PEF-induced Ca influx to mammalian cells
  Kazuyuki Kitera, Shin Furukawa, Sunao Katsuki, Takeshi Ihara
• 16:30–16:45 O144 Effects of Ultra-fast Nanosecond Pulses on Mitochondria and the Efficacy of Calcium Electrochemotherapy with Gold Nanoparticles
  Paulina Malakauskaitė, Eglė Mickevičiūtė-Zinkuvienė, Rokas Žalnėravičius, Vytautas Kašėta, Vitalij Novickij
• 16:45–17:00 O145 In Vivo Efficacy and Immune Profiling of Unipolar and Bipolar Nanosecond CaECT
  Veronika Malyško, Eivina Radzevičiūtė-Valčiukė, Augustinas Želvys, Auksė Zinkevičienė, Vytautas Kašėta, Joanna Rossowska, Julita Kulbacka, Vitalij Novickij
• 17:00–17:15 O146 Contactless HI-PEMF Magnetoporation: In Vivo Proof of Concept of Calcium and Bleomycin Magnetochemotherapies
  Augustinas Želvys, Eglė Mickevičiūtė-Zinkuvienė, Paulina Malakauskaitė, Barbora Lekešytė, Vytautas Kašėta, Vitalij Novickij
• 17:15–17:30 O147 Effects of Calcium Depletion in the Early Events of Nanoseocond Pulsed Electric Field (nsPEF)-induced Cell Differentiation of HL-60 Cells
  Ellen Jing, Iurii Semenov, Siqi Guo

• 16:15–16:30 O133 Senescence-Associated Changes in Membrane Lipid Order and Cytoskeleton Modulate Susceptibility to Pulsed Electric Field Electroporation
  Ippei Yagi, Satoshi Uchida
• 16:30–16:45 O134 Unexpected fate of spherical gold nanoparticles following electroporation
  Emma Barrère, Nicolas Mattei, Ophélie Cordier, Muriel Golzio, Elisabetta Sieni, Diana Ciuculescu Pradines, Marie-Pierre Rols, Jelena Kolosnjaj-Tabi
• 16:45–17:00 O135 Toward Clinical Magnetic Resonance-Guided Irreversible Electroporation: In-bore IRE Demonstrated in a Porcine Model
  Othmar Belker, Simon Schröer, Thomas Gerlach, Max Joris Hubmann, Daniel Düx, Marcel Eisenmann, Christoph Fritsch, Julian Glandorf, Seben Sena Yerdelen, Inga Bruesch, Björn Hartleben, Sahar Arbabzadah, Jörg Mengwasser, Philipp Felgendreff, Georg Rose, Frank Wacker, Marcel Gutberlet, Bennet Hensen
• 17:00–17:15 O136 Characterization of Cardiac Myoblast Electroporation Thresholds: Influence of Cell Geometry, H-FIRE Waveforms, and Ion-Induced Resilience
  Aras Rafanavičius, Ernestas Urbanskas, Saulius Šatkauskas
• 17:15–17:30 O137 Electrofusion of Adherent A549 Cells Through High-Frequency Pulsed Electric Fields
  Stephanie Hallerman, Neeraj Raghuraman Rajagopalan, Fatema Mandsaurwala, Govind Srimathveeravalli

• 08:30–09:00 PT9 H-FIRE in Canine Liver Cancer: Treatment Delivery, Tissue Response, and Translational Insights
  Nikolaos Dervisis
• 09:00–09:30 PT10 Effects of Electric Fields on Vegetative Bacterial Cells, Bacterial Spores and Enzymes
  Sudhir Sastry

No abstracts in this session.

• 10:45–11:00 O160 Endovascular Electroporation as a Strategy to Reduce Post-Stent Restenosis: A translational Canine Model
  Jesica Miranda, Jorge Garcia, Sebastian D. Michinski, Nazarena Martinez, Gonzalo Anton, Aldo Perusso, Felipe H. Maglietti
• 11:00–11:15 O161 Electrochemotherapy for Lingual Tumors in Dogs and Cats: Technical Considerations and Clinical Outcomes
  Matías N. Tellado, Juan Vizcay, Juan M Fernández, Marina Riveiro, Felipe H. Maglietti
• 11:15–11:30 O162 Pharmacokinetics of Bleomycin in Serum and Tumour Tissue of Dogs Treated with Electrochemotherapy
  Nina Milevoj, Ursa Lampreht Tratar, Tina Kosjek, Mojca Krzan, Filippo Torrigiani, Gregor Sersa, Maja Cemazar
• 11:30–11:45 O163 VetInspECT: A Platform for Multicentre Standardisation of Electrochemotherapy Outcomes in Veterinary Oncology—Results from Feline Tumors
  Nataša Tozon, Ursa Lampreht Tratar, Nina Milevoj, Francesca De Terlizzi, Tanya Birk, Gregor Sersa, Maja Cemazar
• 11:45–12:00 O164 Percutaneous intramyocardial septal ablation with nanosecond pulsed electric fields in canine model
  Zhihong Zhao, Xinghua Chen, Zhaoxia Pu, Xiangyang Xia, Yonggang Chen, Bin Wu
• 12:00–12:15 O165 Surgical Resection Combined with Intraoperative Electrochemotherapy Using a Tetrapolar Single-Needle Electrode for Intranasal Tumors in Dogs
  Marcelo Monte Mor Rangel, Andre Gustavo Alves Holanda, Daniela Ota Hisayasu Suzuki

• 10:45–11:00 O148 Physical Model for Cell Membrane Surface Conversion from Hydrophilic to Hydrophobic During Electric-Field Pulsing
  Joe McPherson
• 11:00–11:15 O149 Interpreting the Strength–Duration Curve: How Subcellular Charging, Active Membrane Response, and Electroporation Affect Excitability.
  Federica Serra, Christian W. Zemlin
• 11:15–11:30 O150 From quadratic to linear: A revised asymptotic electroporation model
  Rok Šmerc, Lea Rems
• 11:30–11:45 O151 Multiscale Modeling of Electroporation Capturing Long-Term Membrane Permeability
  Emil Timlin, Irina Pettersson, Tobias Gebäck, Clair Poignard
• 11:45–12:00 O152 Single-Cell Patch-Clamp Reveals a Distinct Electrical Phenotype in H2O2-Induced Senescent Cells
  Jiajun Yu, Satoshi Uchida, Ippei Yagi
• 12:00–12:15 O153 DAMP Release and Regrowth Dynamics in Tumour Spheroids Exposed to Pulsed Electric Fields
  Emma Leschiera, Nicolas Mattei, Marie-Pierre Rols, Muriel Golzio, Jelena Kolosnjaj-Tabi, Clair Poignard

• 10:45–11:00 O154 From Microalgae to Jet Fuel: A Low-Energy Strategy for Lipid Recovery Using Combined Autolysis and Pulsed Electric Fields
  Christian Gusbeth, Emine Erdogan Özseker, Wolfgang W. Frey
• 11:00–11:15 O155 Effective Antimicrobial Surfaces Based on Electroporation with Microscale Interdigitated Electrodes
  Xing Xie
• 11:15–11:30 O156 Pulsed Electric Field–Induced Electroporation Modulates Mass Transfer Kinetics and Cultivar-Dependent Biochemical Effects in Fresh-Cut Apples
  Lily Mize, Ashley Johnson, Stephanie Cho, Elijah Roach, Reece Hutchens, Julia Yasenchak, Nicholas Horlacher, Indrawati Oey, Michael Danquah
• 11:30–11:45 O157 Pulsed Electric Field-Assisted Fermentation: a novel strategy for Kombucha Production
  Francesca Pompei, Mattia Acito, Junior Bernardo Molina Hernandez, Silvia Tappi, Iolanda Grappasonni, Sauro Vittori, Cinzia Mannozzi
• 11:45–12:00 O158 Brewing the future: Pulsed electric field applied for cold coffee brewing
  Cinzia Mannozzi, Francesca Pompei, Riccardo Marconi, Federico Drudi, Urszula Tylewicz, Santina Romani, Lauro Fioretti, Sauro Vittori
• 12:00–12:15 O159 Electric Field Pretreatment for Enhanced Anaerobic Digestion of Food Waste
  Mindaugas Visockis, Devu Pengadeth, Ajay Menon, Norman Maloney, James Lyng

No abstracts in this session.

No abstracts in this session.